Understanding and (dis)trusting food assurance schemes::Consumer confidence and the ‘knowledge fix’

This paper uses evidence from focus groups with consumers in England to consider how consumers understand and evaluate a range of proxies or intermediary organisations that offer assurance about food and consumer products, particularly voluntary certification schemes. This addresses the current concern in developed economies about providing information in order to reconnect consumers with food producers and to support moves towards more local, fairly traded and sustainable production. However, we show that such a ‘knowledge fix’ approach of providing information may not reconnect consumers so easily. Participants found it particularly difficult to work out what certification involved and what kinds of organisations were providing assurance. They built vernacular typologies and comparative judgements that did not necessarily identify or prioritise ‘independent’ third-party certification as the gold standard, not least because of the practical difficulties of monitoring complex supply chains, and expressed confusion and scepticism about how well food assurance schemes could work in practice. Our results therefore problematise the knowledge fix urged in the literature and emphasise instead the need to better understand how consumers make sense of assurance information in different contexts

Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish

The zebrafish has become an important model for cancer research. Several cancer models have been established by transgenic expression of human or mouse oncogenes in zebrafish. Since it is amenable to efficient transgenesis, zebrafish have immense potential to be used for studying interaction of oncogenes and pathways at the organismal level. Using the Gal4VP16-UAS binary transgenic expression approach, we established stable transgenic lines expressing an EGFP fusion protein of an activated zebrafish Smoothened (Smoa1-EGFP). Expression of the zebrafish Smoa1-EGFP itself did not lead to tumor formation either in founder fish or subsequent generations, however, co-expressing a constitutively active human AKT1 resulted in several tumor types, including spindle cell sarcoma, rhabdomyoma, ocular melanoma, astrocytoma, and myoxma. All tumor types showed GFP expression and increased Patched 1 levels, suggesting involvement of zebrafish Smoa1 in tumorigenesis. Immunofluorescence studies showed that tumors also expressed elevated levels of phosphorylated AKT, indicating activation of the PI3K-AKT pathway. These results suggest that co-activation of the hedgehog and AKT pathways promote tumorigenesis, and that the binary transgenic approach is a useful tool for studying interaction of oncogenes and oncogenic pathways in zebrafish

Reviews

Stories About Stories: Fantasy and the Remaking of Myth. Brian Attebery. Reviewed by David Bratman. The Body in Tolkien\u27s Legendarium: Essays on Middle-earth Corporeality. Edited by Christopher Vaccaro. Reviewed by Janet Brennan Croft. Critical Essays on Lord Dunsany. S.T. Joshi, ed. Lanham MD. Reviewed by Tiffany Brooke Martin. History, Guilt, and Habit. Owen Barfield. Reviewed by Bradford Lee Eden. In the Nameless Wood: Explorations in the Philological Hinterland of Tolkien\u27s Literary Creations. J.S. Ryan. Edited by Peter Buchs. Reviewed by Andrew Higgins. The Letters of Ruth Pitter: Silent Music. Edited by Don W. King. Reviewed by Joe R. Christopher

Two novel missense mutations in ABCA1 result in altered trafficking and cause severe autosomal recessive HDL deficiency

AbstractExtremely low concentrations of high density lipoprotein (HDL)-cholesterol and apolipoprotein (apo) AI are features of Tangier disease caused by autosomal recessive mutations in ATP-binding cassette transporter A1 (ABCA1). Less deleterious, but dominantly inherited mutations cause HDL deficiency. We investigated causes of severe HDL deficiency in a 42-year-old female with progressive coronary disease.ApoAI-mediated efflux of cholesterol from the proband's fibroblasts was less than 10% of normal and nucleotide sequencing revealed inheritance of two novel mutations in ABCAI, V1704D and L1379F. ABCA1 mRNA was approximately 3-fold higher in the proband's cells than in control cells; preincubation with cholesterol increased it 5-fold in control and 8-fold in the proband's cells, but similar amounts of ABCA1 protein were present in control and mutant cells. When transiently transfected into HEK293 cells, confocal microscopy revealed that both mutant proteins were retained in the endoplasmic reticulum, while wild-type ABCA1 was located at the plasma membrane.Severe HDL deficiency in the proband was caused by two novel autosomal recessive mutations in ABCA1, one (V1704D) predicted to lie in a transmembrane segment and the other (L1379F) in a large extracellular loop. Both mutations prevent normal trafficking of ABCA1, thereby explaining their inability to mediate apoA1-dependent lipid efflux

Allergy and Risk of Childhood Leukaemia: Results from the UKCCS

We investigated the relationship between childhood leukaemia and preceding history of allergy. A nationwide case-control study of childhood cancers was conducted in the United Kingdom with population-based sampling of cases (n = 839) and controls (n = 1,337), matched on age, sex and region of residence. Information about clinically diagnosed allergies was obtained from primary care records. More than a third of subjects had at least one allergy diagnosed prior to leukaemia diagnosis (cases) or pseudo-diagnosis (controls). For both total acute lymphoblastic leukaemia (ALL) and common-ALL/precursor B-cell ALL (c-ALL), a history of eczema was associated with a 30% significant reduction in risk: the odds ratios (OR) and 95% confidence intervals (CI) were 0.70 (0.51-0.97) and 0.68 (0.48-0.98), respectively. Similar associations were observed for hayfever (OR = 0.47; 95% CI: 0.26-0.85 and OR = 0.62; 95% CI: 0.33-1.16 for ALL and c-ALL, respectively). No such patterns were seen either for asthma and ALL, or for any allergy and acute myeloid leukaemia. A comparative analysis of primary care records with parents recall of allergy revealed only moderate agreement with contemporaneous clinical diagnoses for both cases and controls - confirming the unreliability of parental report at interview. Our finding of a reciprocal relationship between allergy and ALL in children is compatible with the hypothesis that a dysregulated immune response is a critical determinant of childhood ALL

High-resolution imaging follow-up of doubly imaged quasars

We report upon three years of follow-up and confirmation of doubly imaged quasar lenses through imaging campaigns from 2016-2018 with the Near-Infrared Camera2 (NIRC2) on the W. M. Keck Observatory. A sample of 57 quasar lens candidates are imaged in adaptive-optics-assisted or seeing-limited $K^\prime$-band observations. Out of these 57 candidates, 15 are confirmed as lenses. We form a sample of 20 lenses adding in a number of previously-known lenses that were imaged with NIRC2 in 2013-14 as part of a pilot study. By modelling these 20 lenses, we obtain $K^\prime$-band relative photometry and astrometry of the quasar images and the lens galaxy. We also provide the lens properties and predicted time delays to aid planning of follow-up observations necessary for various astrophysical applications, e.g., spectroscopic follow-up to obtain the deflector redshifts for the newly confirmed systems. We compare the departure of the observed flux ratios from the smooth-model predictions between doubly and quadruply imaged quasar systems. We find that the departure is consistent between these two types of lenses if the modelling uncertainty is comparable.Comment: 11 pages, 8 figures, 5 tables. This version: accepted to MNRA